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rnascope® ls 4-plex ancillary kit multiplex reagent kit  (Advanced Cell Diagnostics Inc)

 
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    Advanced Cell Diagnostics Inc rnascope® ls 4-plex ancillary kit multiplex reagent kit

    Rnascope® Ls 4 Plex Ancillary Kit Multiplex Reagent Kit, supplied by Advanced Cell Diagnostics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rnascope+ls+4-plex+ancillary+kit+multiplex+reagent+kit/rnascope+multiplex+fluorescent+reagent+kit+v2/pmc08721283-54-0-9
    Average 90 stars, based on 1 article reviews
    rnascope® ls 4-plex ancillary kit multiplex reagent kit - by Bioz Stars, 2026-09
    90/100 stars

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    1) Product Images from "Resolving the immune landscape of human prostate at a single-cell level in health and cancer"

    Article Title: Resolving the immune landscape of human prostate at a single-cell level in health and cancer

    Journal: Cell Reports

    doi: 10.1016/j.celrep.2021.110132


    Figure Legend Snippet:

    Techniques Used: Control, Zinc Assay, Multiplex Assay, RNAscope, Gene Expression, Software

    Related Articles

    RNA In Situ Hybridization:

    Article Title: Resolving the immune landscape of human prostate at a single-cell level in health and cancer
    Article Snippet: Reagent or resource Source Identifier Biological samples Human normal and cancer prostate tissues Cambridge University Hospitals NHS Foundation Trust N/A Critical commercial assays Zinc Assay Kit Abcam Cat#ab102507 Chromium Single Cell 30 Library & Gel Bead Kit v2 10X Genomics Cat#PN-120237 Chromium Single Cell A Chip Kit, 16 rxns 10X Genomics Cat#PN-1000009 Chromium i7 Multiplex Kit, 96 rxns 10X Genomics Cat#PN-120262 RNAscope 2.5 LS Multiplex Reagent Kit Advanced Cell Diagnostics Cat#322800 RNAscope LS 4-Plex Ancillary Kit Multiplex Reagent Kit Advanced Cell Diagnostics Cat#322830 RNAscope 2.5 LS Probe- Hs-CD68-C2 Advanced Cell Diagnostics Cat#560598-C2 RNAscope 2.5 LS Probe- Hs-MT1-C3 (custom probe) Advanced Cell Diagnostics Cat#831088 Deposited data Matched normal and tumor prostate scRNaseq data This paper, European Genome– Phenome Archive EGAS00001005787, prostatecellatlas.org Spatial Gene Expression Dataset by Space Ranger 1.3.0, Human Prostate Cancer, Adenocarcinoma with Invasive Carcinoma (FFPE) 10X Genomics N/A Prostate Cancer Spatial Transcriptomics data (L1.2) (Berglund et al., 2018) European Genome–Phenome Archive EGAS0000100300 Normal human prostate scRNaseq data (Henry et al., 2018) GEO GSE120716 Human prostate cancer scRNaseq data (Chen et al., 2021) GEO GSE141445 Human prostate cancer scRNaseq data (Crowley et al., 2020) GEO GSE150692 Human prostate cancer scRNaseq data (Karthaus et al., 2020) https://singlecell.broadinstitute.org/ SCP864 Mouse prostate scRNaseq data (Karthaus et al., 2020) GEO, https://singlecell.broadinstitute.org/ GSE146811, SCP859 Human prostate cancer Bulk RNaseq TCGAbiolinks (R/Bioconductor) TCGA-PRAD Human spleen and lung scRNaseq data (Madissoon et al., 2019) Human Cell Atlas Data Portal, https://www.tissuestabilitycellatlas.org/ N/A Human kidney scRNaseq data (Stewart et al., 2019) Human Cell Atlas Data Portal, https://www.kidneycellatlas.org/ N/A Human liver scRNaseq data (MacParland et al., 2018) GEO GSE115469 Human heart scRNaseq data (Wang et al., 2020) GEO GSE109816 Experimental models: Organisms/strains Mouse: C57BL/6 (B6) Jackson Laboratories Stock No: 000664 Software and algorithms seurat CRAN V3.2.3 glmnet CRAN V4.1-2 survival CRAN V2.41-3 (Continued on next page) e2 Cell Reports 37, 110132, December 21, 2021 Resource ll OPEN ACCESS

    Article Title: Resolving the immune landscape of human prostate at a single-cell level in health and cancer
    Article Snippet: Briefly, sections were cut at 3 mm thick, baked for 1 h at 60 C before loading onto a Bond RX instrument (Leica Biosystems).

    Article Title: IL ‐7‐dependent compositional changes within the γδ T cell pool in lymph nodes during ageing lead to an unbalanced anti‐tumour response
    Article Snippet: Probes and Kits (RNAscope LS Multiplex Reagent Kit, Cat# 322800 and RNAscope LS 4‐Plex Ancillary Kit Multiplex Reagent Kit Cat# 322830) were obtained from Advanced Cell Diagnostics.

    Formalin-fixed Paraffin-Embedded:

    Article Title: Resolving the immune landscape of human prostate at a single-cell level in health and cancer
    Article Snippet: Reagent or resource Source Identifier Biological samples Human normal and cancer prostate tissues Cambridge University Hospitals NHS Foundation Trust N/A Critical commercial assays Zinc Assay Kit Abcam Cat#ab102507 Chromium Single Cell 30 Library & Gel Bead Kit v2 10X Genomics Cat#PN-120237 Chromium Single Cell A Chip Kit, 16 rxns 10X Genomics Cat#PN-1000009 Chromium i7 Multiplex Kit, 96 rxns 10X Genomics Cat#PN-120262 RNAscope 2.5 LS Multiplex Reagent Kit Advanced Cell Diagnostics Cat#322800 RNAscope LS 4-Plex Ancillary Kit Multiplex Reagent Kit Advanced Cell Diagnostics Cat#322830 RNAscope 2.5 LS Probe- Hs-CD68-C2 Advanced Cell Diagnostics Cat#560598-C2 RNAscope 2.5 LS Probe- Hs-MT1-C3 (custom probe) Advanced Cell Diagnostics Cat#831088 Deposited data Matched normal and tumor prostate scRNaseq data This paper, European Genome– Phenome Archive EGAS00001005787, prostatecellatlas.org Spatial Gene Expression Dataset by Space Ranger 1.3.0, Human Prostate Cancer, Adenocarcinoma with Invasive Carcinoma (FFPE) 10X Genomics N/A Prostate Cancer Spatial Transcriptomics data (L1.2) (Berglund et al., 2018) European Genome–Phenome Archive EGAS0000100300 Normal human prostate scRNaseq data (Henry et al., 2018) GEO GSE120716 Human prostate cancer scRNaseq data (Chen et al., 2021) GEO GSE141445 Human prostate cancer scRNaseq data (Crowley et al., 2020) GEO GSE150692 Human prostate cancer scRNaseq data (Karthaus et al., 2020) https://singlecell.broadinstitute.org/ SCP864 Mouse prostate scRNaseq data (Karthaus et al., 2020) GEO, https://singlecell.broadinstitute.org/ GSE146811, SCP859 Human prostate cancer Bulk RNaseq TCGAbiolinks (R/Bioconductor) TCGA-PRAD Human spleen and lung scRNaseq data (Madissoon et al., 2019) Human Cell Atlas Data Portal, https://www.tissuestabilitycellatlas.org/ N/A Human kidney scRNaseq data (Stewart et al., 2019) Human Cell Atlas Data Portal, https://www.kidneycellatlas.org/ N/A Human liver scRNaseq data (MacParland et al., 2018) GEO GSE115469 Human heart scRNaseq data (Wang et al., 2020) GEO GSE109816 Experimental models: Organisms/strains Mouse: C57BL/6 (B6) Jackson Laboratories Stock No: 000664 Software and algorithms seurat CRAN V3.2.3 glmnet CRAN V4.1-2 survival CRAN V2.41-3 (Continued on next page) e2 Cell Reports 37, 110132, December 21, 2021 Resource ll OPEN ACCESS

    Article Title: Resolving the immune landscape of human prostate at a single-cell level in health and cancer
    Article Snippet: Briefly, sections were cut at 3 mm thick, baked for 1 h at 60 C before loading onto a Bond RX instrument (Leica Biosystems).

    Article Title: IL ‐7‐dependent compositional changes within the γδ T cell pool in lymph nodes during ageing lead to an unbalanced anti‐tumour response
    Article Snippet: Probes and Kits (RNAscope LS Multiplex Reagent Kit, Cat# 322800 and RNAscope LS 4‐Plex Ancillary Kit Multiplex Reagent Kit Cat# 322830) were obtained from Advanced Cell Diagnostics.

    Multiplex Assay:

    Article Title: Resolving the immune landscape of human prostate at a single-cell level in health and cancer
    Article Snippet: Reagent or resource Source Identifier Biological samples Human normal and cancer prostate tissues Cambridge University Hospitals NHS Foundation Trust N/A Critical commercial assays Zinc Assay Kit Abcam Cat#ab102507 Chromium Single Cell 30 Library & Gel Bead Kit v2 10X Genomics Cat#PN-120237 Chromium Single Cell A Chip Kit, 16 rxns 10X Genomics Cat#PN-1000009 Chromium i7 Multiplex Kit, 96 rxns 10X Genomics Cat#PN-120262 RNAscope 2.5 LS Multiplex Reagent Kit Advanced Cell Diagnostics Cat#322800 RNAscope LS 4-Plex Ancillary Kit Multiplex Reagent Kit Advanced Cell Diagnostics Cat#322830 RNAscope 2.5 LS Probe- Hs-CD68-C2 Advanced Cell Diagnostics Cat#560598-C2 RNAscope 2.5 LS Probe- Hs-MT1-C3 (custom probe) Advanced Cell Diagnostics Cat#831088 Deposited data Matched normal and tumor prostate scRNaseq data This paper, European Genome– Phenome Archive EGAS00001005787, prostatecellatlas.org Spatial Gene Expression Dataset by Space Ranger 1.3.0, Human Prostate Cancer, Adenocarcinoma with Invasive Carcinoma (FFPE) 10X Genomics N/A Prostate Cancer Spatial Transcriptomics data (L1.2) (Berglund et al., 2018) European Genome–Phenome Archive EGAS0000100300 Normal human prostate scRNaseq data (Henry et al., 2018) GEO GSE120716 Human prostate cancer scRNaseq data (Chen et al., 2021) GEO GSE141445 Human prostate cancer scRNaseq data (Crowley et al., 2020) GEO GSE150692 Human prostate cancer scRNaseq data (Karthaus et al., 2020) https://singlecell.broadinstitute.org/ SCP864 Mouse prostate scRNaseq data (Karthaus et al., 2020) GEO, https://singlecell.broadinstitute.org/ GSE146811, SCP859 Human prostate cancer Bulk RNaseq TCGAbiolinks (R/Bioconductor) TCGA-PRAD Human spleen and lung scRNaseq data (Madissoon et al., 2019) Human Cell Atlas Data Portal, https://www.tissuestabilitycellatlas.org/ N/A Human kidney scRNaseq data (Stewart et al., 2019) Human Cell Atlas Data Portal, https://www.kidneycellatlas.org/ N/A Human liver scRNaseq data (MacParland et al., 2018) GEO GSE115469 Human heart scRNaseq data (Wang et al., 2020) GEO GSE109816 Experimental models: Organisms/strains Mouse: C57BL/6 (B6) Jackson Laboratories Stock No: 000664 Software and algorithms seurat CRAN V3.2.3 glmnet CRAN V4.1-2 survival CRAN V2.41-3 (Continued on next page) e2 Cell Reports 37, 110132, December 21, 2021 Resource ll OPEN ACCESS

    Article Title: Resolving the immune landscape of human prostate at a single-cell level in health and cancer
    Article Snippet: Briefly, sections were cut at 3 mm thick, baked for 1 h at 60 C before loading onto a Bond RX instrument (Leica Biosystems).

    Article Title: IL ‐7‐dependent compositional changes within the γδ T cell pool in lymph nodes during ageing lead to an unbalanced anti‐tumour response
    Article Snippet: Probes and Kits (RNAscope LS Multiplex Reagent Kit, Cat# 322800 and RNAscope LS 4‐Plex Ancillary Kit Multiplex Reagent Kit Cat# 322830) were obtained from Advanced Cell Diagnostics.

    Histopathology:

    Article Title: Resolving the immune landscape of human prostate at a single-cell level in health and cancer
    Article Snippet: Reagent or resource Source Identifier Biological samples Human normal and cancer prostate tissues Cambridge University Hospitals NHS Foundation Trust N/A Critical commercial assays Zinc Assay Kit Abcam Cat#ab102507 Chromium Single Cell 30 Library & Gel Bead Kit v2 10X Genomics Cat#PN-120237 Chromium Single Cell A Chip Kit, 16 rxns 10X Genomics Cat#PN-1000009 Chromium i7 Multiplex Kit, 96 rxns 10X Genomics Cat#PN-120262 RNAscope 2.5 LS Multiplex Reagent Kit Advanced Cell Diagnostics Cat#322800 RNAscope LS 4-Plex Ancillary Kit Multiplex Reagent Kit Advanced Cell Diagnostics Cat#322830 RNAscope 2.5 LS Probe- Hs-CD68-C2 Advanced Cell Diagnostics Cat#560598-C2 RNAscope 2.5 LS Probe- Hs-MT1-C3 (custom probe) Advanced Cell Diagnostics Cat#831088 Deposited data Matched normal and tumor prostate scRNaseq data This paper, European Genome– Phenome Archive EGAS00001005787, prostatecellatlas.org Spatial Gene Expression Dataset by Space Ranger 1.3.0, Human Prostate Cancer, Adenocarcinoma with Invasive Carcinoma (FFPE) 10X Genomics N/A Prostate Cancer Spatial Transcriptomics data (L1.2) (Berglund et al., 2018) European Genome–Phenome Archive EGAS0000100300 Normal human prostate scRNaseq data (Henry et al., 2018) GEO GSE120716 Human prostate cancer scRNaseq data (Chen et al., 2021) GEO GSE141445 Human prostate cancer scRNaseq data (Crowley et al., 2020) GEO GSE150692 Human prostate cancer scRNaseq data (Karthaus et al., 2020) https://singlecell.broadinstitute.org/ SCP864 Mouse prostate scRNaseq data (Karthaus et al., 2020) GEO, https://singlecell.broadinstitute.org/ GSE146811, SCP859 Human prostate cancer Bulk RNaseq TCGAbiolinks (R/Bioconductor) TCGA-PRAD Human spleen and lung scRNaseq data (Madissoon et al., 2019) Human Cell Atlas Data Portal, https://www.tissuestabilitycellatlas.org/ N/A Human kidney scRNaseq data (Stewart et al., 2019) Human Cell Atlas Data Portal, https://www.kidneycellatlas.org/ N/A Human liver scRNaseq data (MacParland et al., 2018) GEO GSE115469 Human heart scRNaseq data (Wang et al., 2020) GEO GSE109816 Experimental models: Organisms/strains Mouse: C57BL/6 (B6) Jackson Laboratories Stock No: 000664 Software and algorithms seurat CRAN V3.2.3 glmnet CRAN V4.1-2 survival CRAN V2.41-3 (Continued on next page) e2 Cell Reports 37, 110132, December 21, 2021 Resource ll OPEN ACCESS

    Article Title: Resolving the immune landscape of human prostate at a single-cell level in health and cancer
    Article Snippet: Briefly, sections were cut at 3 mm thick, baked for 1 h at 60 C before loading onto a Bond RX instrument (Leica Biosystems).

    Article Title: IL ‐7‐dependent compositional changes within the γδ T cell pool in lymph nodes during ageing lead to an unbalanced anti‐tumour response
    Article Snippet: Probes and Kits (RNAscope LS Multiplex Reagent Kit, Cat# 322800 and RNAscope LS 4‐Plex Ancillary Kit Multiplex Reagent Kit Cat# 322830) were obtained from Advanced Cell Diagnostics.

    Hybridization:

    Article Title: Resolving the immune landscape of human prostate at a single-cell level in health and cancer
    Article Snippet: Reagent or resource Source Identifier Biological samples Human normal and cancer prostate tissues Cambridge University Hospitals NHS Foundation Trust N/A Critical commercial assays Zinc Assay Kit Abcam Cat#ab102507 Chromium Single Cell 30 Library & Gel Bead Kit v2 10X Genomics Cat#PN-120237 Chromium Single Cell A Chip Kit, 16 rxns 10X Genomics Cat#PN-1000009 Chromium i7 Multiplex Kit, 96 rxns 10X Genomics Cat#PN-120262 RNAscope 2.5 LS Multiplex Reagent Kit Advanced Cell Diagnostics Cat#322800 RNAscope LS 4-Plex Ancillary Kit Multiplex Reagent Kit Advanced Cell Diagnostics Cat#322830 RNAscope 2.5 LS Probe- Hs-CD68-C2 Advanced Cell Diagnostics Cat#560598-C2 RNAscope 2.5 LS Probe- Hs-MT1-C3 (custom probe) Advanced Cell Diagnostics Cat#831088 Deposited data Matched normal and tumor prostate scRNaseq data This paper, European Genome– Phenome Archive EGAS00001005787, prostatecellatlas.org Spatial Gene Expression Dataset by Space Ranger 1.3.0, Human Prostate Cancer, Adenocarcinoma with Invasive Carcinoma (FFPE) 10X Genomics N/A Prostate Cancer Spatial Transcriptomics data (L1.2) (Berglund et al., 2018) European Genome–Phenome Archive EGAS0000100300 Normal human prostate scRNaseq data (Henry et al., 2018) GEO GSE120716 Human prostate cancer scRNaseq data (Chen et al., 2021) GEO GSE141445 Human prostate cancer scRNaseq data (Crowley et al., 2020) GEO GSE150692 Human prostate cancer scRNaseq data (Karthaus et al., 2020) https://singlecell.broadinstitute.org/ SCP864 Mouse prostate scRNaseq data (Karthaus et al., 2020) GEO, https://singlecell.broadinstitute.org/ GSE146811, SCP859 Human prostate cancer Bulk RNaseq TCGAbiolinks (R/Bioconductor) TCGA-PRAD Human spleen and lung scRNaseq data (Madissoon et al., 2019) Human Cell Atlas Data Portal, https://www.tissuestabilitycellatlas.org/ N/A Human kidney scRNaseq data (Stewart et al., 2019) Human Cell Atlas Data Portal, https://www.kidneycellatlas.org/ N/A Human liver scRNaseq data (MacParland et al., 2018) GEO GSE115469 Human heart scRNaseq data (Wang et al., 2020) GEO GSE109816 Experimental models: Organisms/strains Mouse: C57BL/6 (B6) Jackson Laboratories Stock No: 000664 Software and algorithms seurat CRAN V3.2.3 glmnet CRAN V4.1-2 survival CRAN V2.41-3 (Continued on next page) e2 Cell Reports 37, 110132, December 21, 2021 Resource ll OPEN ACCESS

    Article Title: Resolving the immune landscape of human prostate at a single-cell level in health and cancer
    Article Snippet: Briefly, sections were cut at 3 mm thick, baked for 1 h at 60 C before loading onto a Bond RX instrument (Leica Biosystems).

    Article Title: IL ‐7‐dependent compositional changes within the γδ T cell pool in lymph nodes during ageing lead to an unbalanced anti‐tumour response
    Article Snippet: Probes and Kits (RNAscope LS Multiplex Reagent Kit, Cat# 322800 and RNAscope LS 4‐Plex Ancillary Kit Multiplex Reagent Kit Cat# 322830) were obtained from Advanced Cell Diagnostics.

    Zinc Assay:

    Article Title: Resolving the immune landscape of human prostate at a single-cell level in health and cancer
    Article Snippet: Reagent or resource Source Identifier Biological samples Human normal and cancer prostate tissues Cambridge University Hospitals NHS Foundation Trust N/A Critical commercial assays Zinc Assay Kit Abcam Cat#ab102507 Chromium Single Cell 30 Library & Gel Bead Kit v2 10X Genomics Cat#PN-120237 Chromium Single Cell A Chip Kit, 16 rxns 10X Genomics Cat#PN-1000009 Chromium i7 Multiplex Kit, 96 rxns 10X Genomics Cat#PN-120262 RNAscope 2.5 LS Multiplex Reagent Kit Advanced Cell Diagnostics Cat#322800 RNAscope LS 4-Plex Ancillary Kit Multiplex Reagent Kit Advanced Cell Diagnostics Cat#322830 RNAscope 2.5 LS Probe- Hs-CD68-C2 Advanced Cell Diagnostics Cat#560598-C2 RNAscope 2.5 LS Probe- Hs-MT1-C3 (custom probe) Advanced Cell Diagnostics Cat#831088 Deposited data Matched normal and tumor prostate scRNaseq data This paper, European Genome– Phenome Archive EGAS00001005787, prostatecellatlas.org Spatial Gene Expression Dataset by Space Ranger 1.3.0, Human Prostate Cancer, Adenocarcinoma with Invasive Carcinoma (FFPE) 10X Genomics N/A Prostate Cancer Spatial Transcriptomics data (L1.2) (Berglund et al., 2018) European Genome–Phenome Archive EGAS0000100300 Normal human prostate scRNaseq data (Henry et al., 2018) GEO GSE120716 Human prostate cancer scRNaseq data (Chen et al., 2021) GEO GSE141445 Human prostate cancer scRNaseq data (Crowley et al., 2020) GEO GSE150692 Human prostate cancer scRNaseq data (Karthaus et al., 2020) https://singlecell.broadinstitute.org/ SCP864 Mouse prostate scRNaseq data (Karthaus et al., 2020) GEO, https://singlecell.broadinstitute.org/ GSE146811, SCP859 Human prostate cancer Bulk RNaseq TCGAbiolinks (R/Bioconductor) TCGA-PRAD Human spleen and lung scRNaseq data (Madissoon et al., 2019) Human Cell Atlas Data Portal, https://www.tissuestabilitycellatlas.org/ N/A Human kidney scRNaseq data (Stewart et al., 2019) Human Cell Atlas Data Portal, https://www.kidneycellatlas.org/ N/A Human liver scRNaseq data (MacParland et al., 2018) GEO GSE115469 Human heart scRNaseq data (Wang et al., 2020) GEO GSE109816 Experimental models: Organisms/strains Mouse: C57BL/6 (B6) Jackson Laboratories Stock No: 000664 Software and algorithms seurat CRAN V3.2.3 glmnet CRAN V4.1-2 survival CRAN V2.41-3 (Continued on next page) e2 Cell Reports 37, 110132, December 21, 2021 Resource ll OPEN ACCESS

    Article Title: Resolving the immune landscape of human prostate at a single-cell level in health and cancer
    Article Snippet: Briefly, sections were cut at 3 mm thick, baked for 1 h at 60 C before loading onto a Bond RX instrument (Leica Biosystems).

    Article Title: IL ‐7‐dependent compositional changes within the γδ T cell pool in lymph nodes during ageing lead to an unbalanced anti‐tumour response
    Article Snippet: Probes and Kits (RNAscope LS Multiplex Reagent Kit, Cat# 322800 and RNAscope LS 4‐Plex Ancillary Kit Multiplex Reagent Kit Cat# 322830) were obtained from Advanced Cell Diagnostics.

    Expressing:

    Article Title: Resolving the immune landscape of human prostate at a single-cell level in health and cancer
    Article Snippet: Reagent or resource Source Identifier Biological samples Human normal and cancer prostate tissues Cambridge University Hospitals NHS Foundation Trust N/A Critical commercial assays Zinc Assay Kit Abcam Cat#ab102507 Chromium Single Cell 30 Library & Gel Bead Kit v2 10X Genomics Cat#PN-120237 Chromium Single Cell A Chip Kit, 16 rxns 10X Genomics Cat#PN-1000009 Chromium i7 Multiplex Kit, 96 rxns 10X Genomics Cat#PN-120262 RNAscope 2.5 LS Multiplex Reagent Kit Advanced Cell Diagnostics Cat#322800 RNAscope LS 4-Plex Ancillary Kit Multiplex Reagent Kit Advanced Cell Diagnostics Cat#322830 RNAscope 2.5 LS Probe- Hs-CD68-C2 Advanced Cell Diagnostics Cat#560598-C2 RNAscope 2.5 LS Probe- Hs-MT1-C3 (custom probe) Advanced Cell Diagnostics Cat#831088 Deposited data Matched normal and tumor prostate scRNaseq data This paper, European Genome– Phenome Archive EGAS00001005787, prostatecellatlas.org Spatial Gene Expression Dataset by Space Ranger 1.3.0, Human Prostate Cancer, Adenocarcinoma with Invasive Carcinoma (FFPE) 10X Genomics N/A Prostate Cancer Spatial Transcriptomics data (L1.2) (Berglund et al., 2018) European Genome–Phenome Archive EGAS0000100300 Normal human prostate scRNaseq data (Henry et al., 2018) GEO GSE120716 Human prostate cancer scRNaseq data (Chen et al., 2021) GEO GSE141445 Human prostate cancer scRNaseq data (Crowley et al., 2020) GEO GSE150692 Human prostate cancer scRNaseq data (Karthaus et al., 2020) https://singlecell.broadinstitute.org/ SCP864 Mouse prostate scRNaseq data (Karthaus et al., 2020) GEO, https://singlecell.broadinstitute.org/ GSE146811, SCP859 Human prostate cancer Bulk RNaseq TCGAbiolinks (R/Bioconductor) TCGA-PRAD Human spleen and lung scRNaseq data (Madissoon et al., 2019) Human Cell Atlas Data Portal, https://www.tissuestabilitycellatlas.org/ N/A Human kidney scRNaseq data (Stewart et al., 2019) Human Cell Atlas Data Portal, https://www.kidneycellatlas.org/ N/A Human liver scRNaseq data (MacParland et al., 2018) GEO GSE115469 Human heart scRNaseq data (Wang et al., 2020) GEO GSE109816 Experimental models: Organisms/strains Mouse: C57BL/6 (B6) Jackson Laboratories Stock No: 000664 Software and algorithms seurat CRAN V3.2.3 glmnet CRAN V4.1-2 survival CRAN V2.41-3 (Continued on next page) e2 Cell Reports 37, 110132, December 21, 2021 Resource ll OPEN ACCESS

    Article Title: Resolving the immune landscape of human prostate at a single-cell level in health and cancer
    Article Snippet: Briefly, sections were cut at 3 mm thick, baked for 1 h at 60 C before loading onto a Bond RX instrument (Leica Biosystems).

    Article Title: IL ‐7‐dependent compositional changes within the γδ T cell pool in lymph nodes during ageing lead to an unbalanced anti‐tumour response
    Article Snippet: Probes and Kits (RNAscope LS Multiplex Reagent Kit, Cat# 322800 and RNAscope LS 4‐Plex Ancillary Kit Multiplex Reagent Kit Cat# 322830) were obtained from Advanced Cell Diagnostics.

    Software:

    Article Title: Resolving the immune landscape of human prostate at a single-cell level in health and cancer
    Article Snippet: Reagent or resource Source Identifier Biological samples Human normal and cancer prostate tissues Cambridge University Hospitals NHS Foundation Trust N/A Critical commercial assays Zinc Assay Kit Abcam Cat#ab102507 Chromium Single Cell 30 Library & Gel Bead Kit v2 10X Genomics Cat#PN-120237 Chromium Single Cell A Chip Kit, 16 rxns 10X Genomics Cat#PN-1000009 Chromium i7 Multiplex Kit, 96 rxns 10X Genomics Cat#PN-120262 RNAscope 2.5 LS Multiplex Reagent Kit Advanced Cell Diagnostics Cat#322800 RNAscope LS 4-Plex Ancillary Kit Multiplex Reagent Kit Advanced Cell Diagnostics Cat#322830 RNAscope 2.5 LS Probe- Hs-CD68-C2 Advanced Cell Diagnostics Cat#560598-C2 RNAscope 2.5 LS Probe- Hs-MT1-C3 (custom probe) Advanced Cell Diagnostics Cat#831088 Deposited data Matched normal and tumor prostate scRNaseq data This paper, European Genome– Phenome Archive EGAS00001005787, prostatecellatlas.org Spatial Gene Expression Dataset by Space Ranger 1.3.0, Human Prostate Cancer, Adenocarcinoma with Invasive Carcinoma (FFPE) 10X Genomics N/A Prostate Cancer Spatial Transcriptomics data (L1.2) (Berglund et al., 2018) European Genome–Phenome Archive EGAS0000100300 Normal human prostate scRNaseq data (Henry et al., 2018) GEO GSE120716 Human prostate cancer scRNaseq data (Chen et al., 2021) GEO GSE141445 Human prostate cancer scRNaseq data (Crowley et al., 2020) GEO GSE150692 Human prostate cancer scRNaseq data (Karthaus et al., 2020) https://singlecell.broadinstitute.org/ SCP864 Mouse prostate scRNaseq data (Karthaus et al., 2020) GEO, https://singlecell.broadinstitute.org/ GSE146811, SCP859 Human prostate cancer Bulk RNaseq TCGAbiolinks (R/Bioconductor) TCGA-PRAD Human spleen and lung scRNaseq data (Madissoon et al., 2019) Human Cell Atlas Data Portal, https://www.tissuestabilitycellatlas.org/ N/A Human kidney scRNaseq data (Stewart et al., 2019) Human Cell Atlas Data Portal, https://www.kidneycellatlas.org/ N/A Human liver scRNaseq data (MacParland et al., 2018) GEO GSE115469 Human heart scRNaseq data (Wang et al., 2020) GEO GSE109816 Experimental models: Organisms/strains Mouse: C57BL/6 (B6) Jackson Laboratories Stock No: 000664 Software and algorithms seurat CRAN V3.2.3 glmnet CRAN V4.1-2 survival CRAN V2.41-3 (Continued on next page) e2 Cell Reports 37, 110132, December 21, 2021 Resource ll OPEN ACCESS

    Article Title: Resolving the immune landscape of human prostate at a single-cell level in health and cancer
    Article Snippet: Briefly, sections were cut at 3 mm thick, baked for 1 h at 60 C before loading onto a Bond RX instrument (Leica Biosystems).

    Article Title: IL ‐7‐dependent compositional changes within the γδ T cell pool in lymph nodes during ageing lead to an unbalanced anti‐tumour response
    Article Snippet: Probes and Kits (RNAscope LS Multiplex Reagent Kit, Cat# 322800 and RNAscope LS 4‐Plex Ancillary Kit Multiplex Reagent Kit Cat# 322830) were obtained from Advanced Cell Diagnostics.

    RNAscope:

    Article Title: Resolving the immune landscape of human prostate at a single-cell level in health and cancer
    Article Snippet: Reagent or resource Source Identifier Biological samples Human normal and cancer prostate tissues Cambridge University Hospitals NHS Foundation Trust N/A Critical commercial assays Zinc Assay Kit Abcam Cat#ab102507 Chromium Single Cell 30 Library & Gel Bead Kit v2 10X Genomics Cat#PN-120237 Chromium Single Cell A Chip Kit, 16 rxns 10X Genomics Cat#PN-1000009 Chromium i7 Multiplex Kit, 96 rxns 10X Genomics Cat#PN-120262 RNAscope 2.5 LS Multiplex Reagent Kit Advanced Cell Diagnostics Cat#322800 RNAscope LS 4-Plex Ancillary Kit Multiplex Reagent Kit Advanced Cell Diagnostics Cat#322830 RNAscope 2.5 LS Probe- Hs-CD68-C2 Advanced Cell Diagnostics Cat#560598-C2 RNAscope 2.5 LS Probe- Hs-MT1-C3 (custom probe) Advanced Cell Diagnostics Cat#831088 Deposited data Matched normal and tumor prostate scRNaseq data This paper, European Genome– Phenome Archive EGAS00001005787, prostatecellatlas.org Spatial Gene Expression Dataset by Space Ranger 1.3.0, Human Prostate Cancer, Adenocarcinoma with Invasive Carcinoma (FFPE) 10X Genomics N/A Prostate Cancer Spatial Transcriptomics data (L1.2) (Berglund et al., 2018) European Genome–Phenome Archive EGAS0000100300 Normal human prostate scRNaseq data (Henry et al., 2018) GEO GSE120716 Human prostate cancer scRNaseq data (Chen et al., 2021) GEO GSE141445 Human prostate cancer scRNaseq data (Crowley et al., 2020) GEO GSE150692 Human prostate cancer scRNaseq data (Karthaus et al., 2020) https://singlecell.broadinstitute.org/ SCP864 Mouse prostate scRNaseq data (Karthaus et al., 2020) GEO, https://singlecell.broadinstitute.org/ GSE146811, SCP859 Human prostate cancer Bulk RNaseq TCGAbiolinks (R/Bioconductor) TCGA-PRAD Human spleen and lung scRNaseq data (Madissoon et al., 2019) Human Cell Atlas Data Portal, https://www.tissuestabilitycellatlas.org/ N/A Human kidney scRNaseq data (Stewart et al., 2019) Human Cell Atlas Data Portal, https://www.kidneycellatlas.org/ N/A Human liver scRNaseq data (MacParland et al., 2018) GEO GSE115469 Human heart scRNaseq data (Wang et al., 2020) GEO GSE109816 Experimental models: Organisms/strains Mouse: C57BL/6 (B6) Jackson Laboratories Stock No: 000664 Software and algorithms seurat CRAN V3.2.3 glmnet CRAN V4.1-2 survival CRAN V2.41-3 (Continued on next page) e2 Cell Reports 37, 110132, December 21, 2021 Resource ll OPEN ACCESS

    Article Title: Resolving the immune landscape of human prostate at a single-cell level in health and cancer
    Article Snippet: Briefly, sections were cut at 3 mm thick, baked for 1 h at 60 C before loading onto a Bond RX instrument (Leica Biosystems).

    Article Title: IL ‐7‐dependent compositional changes within the γδ T cell pool in lymph nodes during ageing lead to an unbalanced anti‐tumour response
    Article Snippet: Probes and Kits (RNAscope LS Multiplex Reagent Kit, Cat# 322800 and RNAscope LS 4‐Plex Ancillary Kit Multiplex Reagent Kit Cat# 322830) were obtained from Advanced Cell Diagnostics.



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    Immune landscape of the prostate includes a prostate-specific macrophage subset enriched in metallothionein transcripts (A) UMAP of 793 cells in myeloid compartment after integration of myeloid/MNP cells from n = 10 patients with Henry et al. myeloid/MNP cells. (B) Mean expression dot plot of top five significant marker genes for each myeloid cluster. Marker genes were identified using Wilcoxon rank sum test and p adj < 0.05 was considered statistically significant. Size of circles indicate percentage of cells expressing the gene and increasing color gradient from white to red corresponds to increasing expression value. (C) UMAP plot of predicted MNP clusters in prostate cancer single cell data from ( <xref ref-type=Karthaus et al., 2020 , Chen et al., 2021 , Crowley et al., 2020 ). (D) (top) Representative RNAscope images of probes targeting MT1 family genes (magenta) and CD68 (yellow). ‘L’ indicates lumen. Arrows point to single cells that are marked by both probes in sub-panels i and ii. Scale bar, 20 μm. (bottom) Representative immunofluorescence microscopy images of a human prostate section labeled for metallothionein (α-MT)/isotype control (yellow), HLA-DR (cyan), CD206 (purple) and DAPI (blue). White arrows point to structure displaying colocalization of α-MT with HLA-DR and/or CD206 labeling. Scale bars, 50 μm. (E) Mean expression dot plot of Zinc transporter genes for each myeloid cluster. Size of circles indicate percentage of cells expressing the gene and increasing color gradient from white to red corresponds to increasing expression value. (F) Heatmap of mean AUCell enrichment of F4/80 hi/lo gene sets, corresponding to yolk sac (YS) versus hematopoetic stem-cell (HSC) lineage. Row enrichment value is scaled from 0 to 1 and presented as an increasing gradient from black, gray, yellow to orange which corresponds to increasing enrichment score. (G) Representative immunofluorescence microscopy images of cross sections of mouse prostate labeled for F4/80 (green), MHCII (red), CD11b (blue), CD31 (yellow) and phalloidin. Scale bars, 120 μm. (H) Cell counts per gram of prostate for rat IgG2a isotype or anti-Csf1r antibody (Ab) treated male mice. N = 5 per group. ∗∗∗∗ p < 0.0001; n.s denotes not significant (p > 0.05) (Two-way ANOVA with Tukey’s multiple correction). (I) Zinc concentration of anterior prostate lobe, liver lobe, and kidney from male mice treated with either rat IgG2a isotype control or anti-Csf1r Ab. N = 6 per group. (shown is representative quantification from one of two independent experiments). ∗ p < 0.05; n.s. not significant (Mann-Whitney test). See also . " width="250" height="auto" />
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    Journal: Cell Reports

    Article Title: Resolving the immune landscape of human prostate at a single-cell level in health and cancer

    doi: 10.1016/j.celrep.2021.110132

    Figure Lengend Snippet:

    Article Snippet: RNAscope® LS 4-Plex Ancillary Kit Multiplex Reagent Kit , Advanced Cell Diagnostics , Cat#322830.

    Techniques: Control, Zinc Assay, Multiplex Assay, RNAscope, Gene Expression, Software

    Immune landscape of the prostate includes a prostate-specific macrophage subset enriched in metallothionein transcripts (A) UMAP of 793 cells in myeloid compartment after integration of myeloid/MNP cells from n = 10 patients with Henry et al. myeloid/MNP cells. (B) Mean expression dot plot of top five significant marker genes for each myeloid cluster. Marker genes were identified using Wilcoxon rank sum test and p adj < 0.05 was considered statistically significant. Size of circles indicate percentage of cells expressing the gene and increasing color gradient from white to red corresponds to increasing expression value. (C) UMAP plot of predicted MNP clusters in prostate cancer single cell data from ( <xref ref-type=Karthaus et al., 2020 , Chen et al., 2021 , Crowley et al., 2020 ). (D) (top) Representative RNAscope images of probes targeting MT1 family genes (magenta) and CD68 (yellow). ‘L’ indicates lumen. Arrows point to single cells that are marked by both probes in sub-panels i and ii. Scale bar, 20 μm. (bottom) Representative immunofluorescence microscopy images of a human prostate section labeled for metallothionein (α-MT)/isotype control (yellow), HLA-DR (cyan), CD206 (purple) and DAPI (blue). White arrows point to structure displaying colocalization of α-MT with HLA-DR and/or CD206 labeling. Scale bars, 50 μm. (E) Mean expression dot plot of Zinc transporter genes for each myeloid cluster. Size of circles indicate percentage of cells expressing the gene and increasing color gradient from white to red corresponds to increasing expression value. (F) Heatmap of mean AUCell enrichment of F4/80 hi/lo gene sets, corresponding to yolk sac (YS) versus hematopoetic stem-cell (HSC) lineage. Row enrichment value is scaled from 0 to 1 and presented as an increasing gradient from black, gray, yellow to orange which corresponds to increasing enrichment score. (G) Representative immunofluorescence microscopy images of cross sections of mouse prostate labeled for F4/80 (green), MHCII (red), CD11b (blue), CD31 (yellow) and phalloidin. Scale bars, 120 μm. (H) Cell counts per gram of prostate for rat IgG2a isotype or anti-Csf1r antibody (Ab) treated male mice. N = 5 per group. ∗∗∗∗ p < 0.0001; n.s denotes not significant (p > 0.05) (Two-way ANOVA with Tukey’s multiple correction). (I) Zinc concentration of anterior prostate lobe, liver lobe, and kidney from male mice treated with either rat IgG2a isotype control or anti-Csf1r Ab. N = 6 per group. (shown is representative quantification from one of two independent experiments). ∗ p < 0.05; n.s. not significant (Mann-Whitney test). See also . " width="100%" height="100%">

    Journal: Cell Reports

    Article Title: Resolving the immune landscape of human prostate at a single-cell level in health and cancer

    doi: 10.1016/j.celrep.2021.110132

    Figure Lengend Snippet: Immune landscape of the prostate includes a prostate-specific macrophage subset enriched in metallothionein transcripts (A) UMAP of 793 cells in myeloid compartment after integration of myeloid/MNP cells from n = 10 patients with Henry et al. myeloid/MNP cells. (B) Mean expression dot plot of top five significant marker genes for each myeloid cluster. Marker genes were identified using Wilcoxon rank sum test and p adj < 0.05 was considered statistically significant. Size of circles indicate percentage of cells expressing the gene and increasing color gradient from white to red corresponds to increasing expression value. (C) UMAP plot of predicted MNP clusters in prostate cancer single cell data from ( Karthaus et al., 2020 , Chen et al., 2021 , Crowley et al., 2020 ). (D) (top) Representative RNAscope images of probes targeting MT1 family genes (magenta) and CD68 (yellow). ‘L’ indicates lumen. Arrows point to single cells that are marked by both probes in sub-panels i and ii. Scale bar, 20 μm. (bottom) Representative immunofluorescence microscopy images of a human prostate section labeled for metallothionein (α-MT)/isotype control (yellow), HLA-DR (cyan), CD206 (purple) and DAPI (blue). White arrows point to structure displaying colocalization of α-MT with HLA-DR and/or CD206 labeling. Scale bars, 50 μm. (E) Mean expression dot plot of Zinc transporter genes for each myeloid cluster. Size of circles indicate percentage of cells expressing the gene and increasing color gradient from white to red corresponds to increasing expression value. (F) Heatmap of mean AUCell enrichment of F4/80 hi/lo gene sets, corresponding to yolk sac (YS) versus hematopoetic stem-cell (HSC) lineage. Row enrichment value is scaled from 0 to 1 and presented as an increasing gradient from black, gray, yellow to orange which corresponds to increasing enrichment score. (G) Representative immunofluorescence microscopy images of cross sections of mouse prostate labeled for F4/80 (green), MHCII (red), CD11b (blue), CD31 (yellow) and phalloidin. Scale bars, 120 μm. (H) Cell counts per gram of prostate for rat IgG2a isotype or anti-Csf1r antibody (Ab) treated male mice. N = 5 per group. ∗∗∗∗ p < 0.0001; n.s denotes not significant (p > 0.05) (Two-way ANOVA with Tukey’s multiple correction). (I) Zinc concentration of anterior prostate lobe, liver lobe, and kidney from male mice treated with either rat IgG2a isotype control or anti-Csf1r Ab. N = 6 per group. (shown is representative quantification from one of two independent experiments). ∗ p < 0.05; n.s. not significant (Mann-Whitney test). See also .

    Article Snippet: Simultaneous detection of human CD68 and MT1 family genes were performed on FFPE sections using Advanced Cell Diagnostics (ACD) RNAscope® 2.5 LS Multiplex Reagent Kit (Cat No. 322800), RNAscope® LS 4-Plex Ancillary Kit Multiplex Reagent Kit (Cat No. 322830), RNAscope® 2.5 LS Probes (ACD, Hayward, CA, USA) at the histopathology/ in situ hybridization core facility at Cancer Research UK – Cambridge Institute.

    Techniques: Expressing, Marker, Immunofluorescence, Microscopy, Labeling, Concentration Assay, MANN-WHITNEY

    Journal: Cell Reports

    Article Title: Resolving the immune landscape of human prostate at a single-cell level in health and cancer

    doi: 10.1016/j.celrep.2021.110132

    Figure Lengend Snippet:

    Article Snippet: Simultaneous detection of human CD68 and MT1 family genes were performed on FFPE sections using Advanced Cell Diagnostics (ACD) RNAscope® 2.5 LS Multiplex Reagent Kit (Cat No. 322800), RNAscope® LS 4-Plex Ancillary Kit Multiplex Reagent Kit (Cat No. 322830), RNAscope® 2.5 LS Probes (ACD, Hayward, CA, USA) at the histopathology/ in situ hybridization core facility at Cancer Research UK – Cambridge Institute.

    Techniques: Zinc Assay, Multiplex Assay, Expressing, Software